Skip to main content
Log in

Heterologous expression of an alginate lyase from Streptomyces sp. ALG-5 in Escherichia coli and its use for preparation of the magnetic nanoparticle-immobilized enzymes

  • Original Paper
  • Published:
Bioprocess and Biosystems Engineering Aims and scope Submit manuscript

Abstract

The marine alginate lyase from Streptomyces sp. ALG-5, which specifically degrades poly-G block of alginate, was functionally expressed as a His-tagged form with an Escherichia coli expression system. The recombinant alginate lyase expressed with pColdI at 15 °C exhibited the highest alginate-degrading activity. The recombinant alginate lyase was efficiently immobilized onto two types of magnetic nanoparticles, superparamagnetic iron oxide nanoparticle, and hybrid magnetic silica nanoparticle, based on the affinity between His-tag and Ni2+ that displayed on the surfaces of nanoparticles. An alginate oligosaccharide mixture consisting of dimer and trimer was prepared by the immobilized alginate lyase. The immobilized enzymes were re-used repeatedly more than 10 times after magnetic separation.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Wong TY, Preston LA, Schiller NL (2000) Alginate lyase: review of major sources and enzyme characteristics, structure-function analysis, biological roles, and application. Annu Rev Microbiol 54:289–340

    Article  CAS  Google Scholar 

  2. Kim DE, Lee EY, Kim HS (2009) Cloning and characterization of alginate lyase from a marine bacterium Streptomyces sp. ALG-5. Mar Biotechnol 11:10–16

    Article  CAS  Google Scholar 

  3. Zhang Z, Yu G, Guan H, Zhao X, Du Y, Jiang X (2004) Preparation and structure elucidation of alginate oligosaccharides degraded by alginate lyase from Vibrio sp. 510. Carbohydr Res 258:187–197

    Google Scholar 

  4. Kawamoto H, Horibe A, Miki Y, Kimura T, Tanaka K, Nakagawa T, Kawamukai M, Matsuda H (2006) Cloning and sequencing analysis of alginate lyase genes from the marine bacterium Vibrio sp. O2. Mar Biotechnol 8:481–490

    Article  CAS  Google Scholar 

  5. Matsubara Y, Kawada R, Iwasaki K, Kimura Y, Oda T, Muramatsu T (2000) Cloning and sequence analysis of a gene (aly PG) encoding poly(α-l-guluronate) lyase from Corynebacterium sp. strain ALY-1. J Biosci Bioeng 89:199–202

    Article  CAS  Google Scholar 

  6. Osawa T, Matsubara Y, Muramatsu T, Kimura M, Kakuta Y (2005) Crystal structure of the alginate (poly-α-l-guluronate) lyase from Corynebacterium sp. at 1.2 Å resolution. J Mol Biol 345:1111–1118

    Article  CAS  Google Scholar 

  7. Iwamoto M, Kurachi M, Nakashima T, Kim D, Yamaguch K, Oda T, Iwamoto Y, Muramatsu T (2005) Structure–activity relationship of alginate oligosaccharides in the induction of cytokine production from RAW264.7 cells. FEBS Lett 579:4423–4429

    Article  CAS  Google Scholar 

  8. Kawada A, Hiura N, Tajima S, Takahara H (1999) Alginate oligosaccharides stimulate VEGF-mediated growth and migration of human endothelial cells. Arch Dermatol Res 291:542–547

    Article  CAS  Google Scholar 

  9. Cao L, Xie L, Xue X, Tan H, Liu Y, Zhou S (2007) Purification and characterization of alginate lyase from Streptomyces species strain A5 isolated from Banana Rhizosphere. J Agric Food Chem 55:5113–5117

    Article  CAS  Google Scholar 

  10. Gimmestad M, Ertesvåg H, Heggeset TMB, Aarstad O, Svanem BIG, Valla S (2009) Characterization of three new Azotobacter vinelandii alginate lyases, one of which is involved in cyst germination. J Bacteriol 191:4845–4853

    Article  CAS  Google Scholar 

  11. Alkawash MA, Soothill JS, Schiller NL (2006) Alginate lyase enhances antibiotic killing of mucoid Pseudomonas aeruginosa in biofilms. APMIS 114:131–138

    Article  CAS  Google Scholar 

  12. Chisti Y (2008) Biodiesel from microalgae beats bioethanol. Trends Biotechnol 26:126–131

    Article  CAS  Google Scholar 

  13. Beer LL, Boyd ES, Peters JW, Posewitz MC (2009) Engineering algae for biohydrogen and biofuel production. Curr Opin Biotechnol 20:264–271

    Article  CAS  Google Scholar 

  14. Vasudevan PT, Briggs M (2008) Biodiesel production-current state of the art and challenges. J Ind Microbiol Biotechnol 35:421–430

    Article  CAS  Google Scholar 

  15. Choi D, Ryu B-Y, Piao YL, Choi S-K, Jo B-W, Shin W-S, Cho H (2008) Studies on saccharification from alginate using Stenotrophomonas maltophilia. J Ind Eng Chem 14:182–186

    CAS  Google Scholar 

  16. Lee IS, Lee N, Park J, Kim BH, Yi YW, Kim T, Kim TK, Lee IH, Paik SR, Hyeon T (2006) Ni/NiO core/shell nanoparticles for selective binding and magnetic separation of histidine. J Am Chem Soc 128:10658–10659

    Article  CAS  Google Scholar 

  17. Lee KS, Lee IS (2008) Decoration of superparamagnetic iron oxide nanoparticles with Ni2+: agent to bind and separate histidine-tagged proteins. Chem Commun 2008:709–711

    Article  Google Scholar 

  18. Lee KS, Woo MH, Kim HS, Lee EY, Lee IS (2009) Synthesis of hybrid Fe3O4/silica/NiO superstructures and their application as magnetically separable high-performance biocatalysts. Chem Commun 25:3780–3782

    Article  Google Scholar 

  19. Yoon H-J, Hashimoto W, Miyake O, Okamoto M, Mikami B, Murata K (2000) Overexpression in Escherichia coli, purification, and characterization of Sphingomonas sp. A1 alginate lyase. Protein Expr Purif 19:84–90

    Article  CAS  Google Scholar 

  20. Jana S, Deb JK (2005) Strategies for efficient production of heterologous proteins in Escherichia coli. Appl Microbiol Biotechnol 67:289–298

    Article  CAS  Google Scholar 

  21. Schlieker C, Bukau B, Mogk A (2002) Prevention and reversion of protein aggregation by molecular chaperones in the E. coli cytosol: implications for their applicability in biotechnology. J Biotechnol 96:13–21

    Article  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by New & Renewable Energy R&D program (20093020090020) under the Korea Ministry of Knowledge Economy (MKE).

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Eun Yeol Lee.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Shin, J.W., Choi, S.H., Kim, D.E. et al. Heterologous expression of an alginate lyase from Streptomyces sp. ALG-5 in Escherichia coli and its use for preparation of the magnetic nanoparticle-immobilized enzymes. Bioprocess Biosyst Eng 34, 113–119 (2011). https://doi.org/10.1007/s00449-010-0452-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00449-010-0452-4

Keywords

Navigation