Abstract
The marine alginate lyase from Streptomyces sp. ALG-5, which specifically degrades poly-G block of alginate, was functionally expressed as a His-tagged form with an Escherichia coli expression system. The recombinant alginate lyase expressed with pColdI at 15 °C exhibited the highest alginate-degrading activity. The recombinant alginate lyase was efficiently immobilized onto two types of magnetic nanoparticles, superparamagnetic iron oxide nanoparticle, and hybrid magnetic silica nanoparticle, based on the affinity between His-tag and Ni2+ that displayed on the surfaces of nanoparticles. An alginate oligosaccharide mixture consisting of dimer and trimer was prepared by the immobilized alginate lyase. The immobilized enzymes were re-used repeatedly more than 10 times after magnetic separation.
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References
Wong TY, Preston LA, Schiller NL (2000) Alginate lyase: review of major sources and enzyme characteristics, structure-function analysis, biological roles, and application. Annu Rev Microbiol 54:289–340
Kim DE, Lee EY, Kim HS (2009) Cloning and characterization of alginate lyase from a marine bacterium Streptomyces sp. ALG-5. Mar Biotechnol 11:10–16
Zhang Z, Yu G, Guan H, Zhao X, Du Y, Jiang X (2004) Preparation and structure elucidation of alginate oligosaccharides degraded by alginate lyase from Vibrio sp. 510. Carbohydr Res 258:187–197
Kawamoto H, Horibe A, Miki Y, Kimura T, Tanaka K, Nakagawa T, Kawamukai M, Matsuda H (2006) Cloning and sequencing analysis of alginate lyase genes from the marine bacterium Vibrio sp. O2. Mar Biotechnol 8:481–490
Matsubara Y, Kawada R, Iwasaki K, Kimura Y, Oda T, Muramatsu T (2000) Cloning and sequence analysis of a gene (aly PG) encoding poly(α-l-guluronate) lyase from Corynebacterium sp. strain ALY-1. J Biosci Bioeng 89:199–202
Osawa T, Matsubara Y, Muramatsu T, Kimura M, Kakuta Y (2005) Crystal structure of the alginate (poly-α-l-guluronate) lyase from Corynebacterium sp. at 1.2 Å resolution. J Mol Biol 345:1111–1118
Iwamoto M, Kurachi M, Nakashima T, Kim D, Yamaguch K, Oda T, Iwamoto Y, Muramatsu T (2005) Structure–activity relationship of alginate oligosaccharides in the induction of cytokine production from RAW264.7 cells. FEBS Lett 579:4423–4429
Kawada A, Hiura N, Tajima S, Takahara H (1999) Alginate oligosaccharides stimulate VEGF-mediated growth and migration of human endothelial cells. Arch Dermatol Res 291:542–547
Cao L, Xie L, Xue X, Tan H, Liu Y, Zhou S (2007) Purification and characterization of alginate lyase from Streptomyces species strain A5 isolated from Banana Rhizosphere. J Agric Food Chem 55:5113–5117
Gimmestad M, Ertesvåg H, Heggeset TMB, Aarstad O, Svanem BIG, Valla S (2009) Characterization of three new Azotobacter vinelandii alginate lyases, one of which is involved in cyst germination. J Bacteriol 191:4845–4853
Alkawash MA, Soothill JS, Schiller NL (2006) Alginate lyase enhances antibiotic killing of mucoid Pseudomonas aeruginosa in biofilms. APMIS 114:131–138
Chisti Y (2008) Biodiesel from microalgae beats bioethanol. Trends Biotechnol 26:126–131
Beer LL, Boyd ES, Peters JW, Posewitz MC (2009) Engineering algae for biohydrogen and biofuel production. Curr Opin Biotechnol 20:264–271
Vasudevan PT, Briggs M (2008) Biodiesel production-current state of the art and challenges. J Ind Microbiol Biotechnol 35:421–430
Choi D, Ryu B-Y, Piao YL, Choi S-K, Jo B-W, Shin W-S, Cho H (2008) Studies on saccharification from alginate using Stenotrophomonas maltophilia. J Ind Eng Chem 14:182–186
Lee IS, Lee N, Park J, Kim BH, Yi YW, Kim T, Kim TK, Lee IH, Paik SR, Hyeon T (2006) Ni/NiO core/shell nanoparticles for selective binding and magnetic separation of histidine. J Am Chem Soc 128:10658–10659
Lee KS, Lee IS (2008) Decoration of superparamagnetic iron oxide nanoparticles with Ni2+: agent to bind and separate histidine-tagged proteins. Chem Commun 2008:709–711
Lee KS, Woo MH, Kim HS, Lee EY, Lee IS (2009) Synthesis of hybrid Fe3O4/silica/NiO superstructures and their application as magnetically separable high-performance biocatalysts. Chem Commun 25:3780–3782
Yoon H-J, Hashimoto W, Miyake O, Okamoto M, Mikami B, Murata K (2000) Overexpression in Escherichia coli, purification, and characterization of Sphingomonas sp. A1 alginate lyase. Protein Expr Purif 19:84–90
Jana S, Deb JK (2005) Strategies for efficient production of heterologous proteins in Escherichia coli. Appl Microbiol Biotechnol 67:289–298
Schlieker C, Bukau B, Mogk A (2002) Prevention and reversion of protein aggregation by molecular chaperones in the E. coli cytosol: implications for their applicability in biotechnology. J Biotechnol 96:13–21
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This work was supported by New & Renewable Energy R&D program (20093020090020) under the Korea Ministry of Knowledge Economy (MKE).
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Shin, J.W., Choi, S.H., Kim, D.E. et al. Heterologous expression of an alginate lyase from Streptomyces sp. ALG-5 in Escherichia coli and its use for preparation of the magnetic nanoparticle-immobilized enzymes. Bioprocess Biosyst Eng 34, 113–119 (2011). https://doi.org/10.1007/s00449-010-0452-4
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DOI: https://doi.org/10.1007/s00449-010-0452-4